Sativa Annam

B

e made up by dried mushroom, containing polyhexose, carrot element, protein, various amino acid, cumarin, nucleotide, increasing the immunity of the children, mainly for the children’s anorexia, cacochylia, infection of the air tube, complexion sensitive, to be taller in a period.

I． Sattva Annam

SATTVA ANNAM is a health food extracted from various edible fungi (those with medicinal functions such as dried mushroom), and produced on the basis of pharmacological testing of such fungi. Two-year clinical observations, and comparisons with TCM treatment for verification, indicate that this health food effectively treats repeated respiratory infection, anorexia, dyspepsia, anemia and related complications
(known as child postinfectious syndrome of weakened spleen) in children. Similar to or even superior to the effectiveness of treatment with medicinal herbs in traditional Chinese medicine, it is easy to use and free from contraindications, allowing complete self-medication.

The main active components of SATTVA ANNAM are polysaccharides from various fungi. The health food also contains active components such as triterpenes, saponins, flavones and propylester.

SATTVA ANNAM serves as a highly effective treatment for repeated respiratory infection, anorexia as well as related complications in children, providing efficient and sustained relief. Since there is currently no efficient or effective treatment domestically available for the diseases, it offers powerful treatment to children. Children using SATTVA ANNAM become more healthy and active, achieving more in their studies and making greater contributions to the community in the future--this is what our researchers hope for.

1. Functions of polysaccharides in edible fungi

Polysaccharides, macromolecular substances made of multiple monosaccharides, are divided into starch, cellulose, dextrin, Tuckahoe polysaccharide, etc. Some polysaccharides are pharmacologically active whereas others are not, depending on the composition and connection of the monosaccharides. Tuckahoe polysaccharide with a (1-4)-connected branched chains tend to be of lower efficiency in enhancing immunity. If the branched chains are disconnected or carboxymethylated to form new Tuckahoe polysaccharides, the power to enhance immunity is greatly improved. Lentinan, the b (1-3) connected glucan, contains no branched chains, leading to higher efficacy in activating T-Lymphocytes. The protein-connecting polysaccharide (known as PSK in Japan) derived from rainbow conk hypha also has glucan with b (1-3) as its main chain, containing 15% protein and a few mannose bases. The polysaccharide promotes the activity of macrophages, thus preventing viral diseases and tumors. The polysaccharide derived from piziza vesicolosa promotes immunity as well as cell division. Prof. Fang Jinian of the Shanghai Drug Research Institute, along with Japanese and Russian researchers, obtained from astragalus the glucan with a (1-4) as its main chain and with branch chain at position 6; this polysaccharide promotes phagocytosis of celiac macrophages, reticuloendothelial cells and T-Lymphocytes.

Polysaccharides of edible fungi are wide-spectrum immunity promoter, varying in functions and features. Medical researches of polysaccharides started in 1943 while the wide-spectrum immunity promoter arouses much interest in 1960s. Prof. Fang points out that more and more research indicates that polysaccharides treat cancers with severely damaged immune systems, immunity deficiency diseases (such as chronic viral hepatitis and drug-resistant bacteria or virus induced chronic diseases), and immunity diseases (such as rheumatism); some also induce interferon. Polysaccharides promote immunity by activating macrophages, T-Lymphocytes and b cells, or by promoting antibody production and activation. It was found that polysaccharides change the structure of a -spiral proteins of bovine serum albumin and decisively affect cell walls, which lead to increased application of polysaccharides upon metabolic disorders, infections and tumors. In recent years, it was found that polysaccharides control cell division and differentiation and regulate cell growth and aging. Inaddition, polysaccharides are an excellent adjuvant.

1. Pharmacological research on the main fungi in the health food for children

The health food, made from various edible fungi such as dried mushrooms, contains the polysaccharides of fungi as its active components. Clinical research reveals that the food effectively treats dyspepsia, weakness, anemia, anorexia, repeated respiratory infection and related complications (referred to by the deputy director of Shanghai TCM Hospital as child postinfectious syndrome of weakened spleen) in children. The health food is warmly received by children and their families. Development of the health food was based on pharmacological tests of the main fungi. In order to protect our findings, we code the main fungi as 8612 and 8645, whose results are as follows:

1. Edible fungus 8612 influence upon animal tolerance to lack of oxygen

Mice weighing from 18 to 20 grams are used in the test, with males and females in half. Apply orally 8612 extract (0.4 ml / 20 g) to the treatment group and saline (0.4 ml / 20 g) to the control group. Thirty minutes later, transfer into wide mouthed bottles with oil-sealed mouths (with 6-gram sodalime inside). Take notes of the breathing cessation time. See the following table.

The test finds out that the 8612 group outlives the control group.

1. Edible fungus 8612 influence upon phagocytosis of macrophages

Take forty pairs of mice and allocate into the treatment group and the control group. Lavage stomach and apply the compound for four successive days. On the third day, apply broth (1 ml/ pair) to the abdominal cavity. On the fourth day, inject 5% chicken red cell liquid (0.5 ml / pair) to the abdominal cavity and put to death one hour later. Make smears with abdominal liquids. See the following table.

The test finds out that the 8612 group promotes phagocytosis of macrophages more effectively than the control group.

1. Edible fungus 8612 fatigue resistance

Take twenty pairs of mice and allocate into the treatment group and the control group. For three successive days, apply the compound abdominally to the treatment group and beef broth to the control group. On the fourth day, half an hour after medication, attach objects to the tail (0.4 g / pair) and put into 25^oC water for swimming. Take notes of swimming time. See the following table.

4. Edible fungus 8612 relief of cough

Mice weighing from 18 to 20 grams are used in the test. Apply the compound orally, one hour later place in closed glass tanks containing ammonia water for one minute. Take out and take notes of the number of coughs in three minutes, with twice as positive and once as half positive. See the following table.

5. Edible fungus 8612 relief of asthma

Guinea pigs weighing from 150 to 250 grams are used in the test. Apply the compound orally, place in spray containers one hour later, spray 0.5% histamine phosphate solution for 30 seconds and observe for incubation period of spasm. Compared with the control group, the incubation period of spasm is 72% longer in the treatment group.

6. Edible fungus 8645 increase of white cells

Take mice of 20 grams and allocate into the treatment group and the control group. Check for WBC count with blood from orbits. Apply the compound to the abdominal cavity beginning on the following day (50-mg medicinal herbs for each mouse) while the control group is given saline. On the seventh day, intraperitoneally inject cyclophosphamide (2 mg / pair). Apply the compound the following day. On the ninth day, check for WBC count again. See the following table.

7. Edible fungus 8645 influence upon appetite of cyclophosphamide-injected mice.

Mice weighing from 18 to 20 grams are divided into the treatment group and the control group. Intraperitoneally inject cyclophosphamide to both groups. Apply 8645 to the treatment group and saline to the control group. It is found that the appetite of the treatment group is definitely higher than that of the control group.

IV. Efficacy study of the health food for children

The efficacy study of the health food is divided into two parts. In the first part, repeated respiratory infection and anorexia as well as related complications are observed. For a better understanding of the efficacy of the health food, the diseases are briefly explained as follows.

Child anorexia: visible lack of appetite of children aged below 12. They appear to have no interest in foods, or lack appetite. They eat much less than the average child of the same age. Some children also appear to have interest in certain foods while having no appetite for most foods. What they eat is also less than the average child of the same age. The cause of anorexia is rather complicated, mainly divided into primary and secondary. No specific reason is found for the primary anorexia (which we study). Most primary anorexia cases are caused by appetite disorders owing to improper feeding, improper dietary habit, eating certain foods only or psychological factors, and a few by poor digestion owing to respiratory infections. Secondary anorexia is caused by appetite disorders owing to stomach, intestinal or other diseases. The health food treats primary anorexia cases and a few secondary anorexia cases.

Child repeated respiratory infection: repeated infection of the upper or lower respiratory tract of children aged below 12. The infections, referred to as respiratory infection, include rhinitis, pharyngitis, laryngitis, tracheitis, bronchitis and pneumonia. Present research indicates that it is because of poor immunity and poor nutrition that a few children are prone to respiratory infections, especially those suffering from deficiency of proteins, vitamin A & D and certain microelements. Infections are closely related to the amount of immunoglobulin SIgA. Lack of the secreted immunoglobulin leads to children respiratory infections. In the study, children who have respiratory infections for at least three successive months are observed. The study proves that the health food safeguards the children by preventing respiratory infections.

Child postinfectious syndrome of weakened spleen: following one or several infections, shown as long-time symptoms of a weakened spleen. The child suffers from continuous lack of appetite, proneness to infections, paleness, emaciation, sweating, fatigue, abnormal excretion (watery or dry stool or constipation), poor sleeping, developmental disorder (for those with long case history), chronic congestion in the throat, enlargement of tonsils and lymph nodes and slight heart murmur. Physical examinations reveal slight anemia, slight rise of WBC count and Neutrophil, lower diastase in urine, lower cellular immunity and humoral immunity, and lower content of copper, zinc and iron in hair.

Efficacy of the health food upon child anorexia and repeated respiratory infection.

1. Clinical observations

Subjects: children aged below 12 suffering from anorexia and repeated respiratory infection. Eighty-five (85) patients were studied, including 47 males and 38 females. 33 patients are below 3 years old, 30 from 4 to 6, 13 from 7 to 9 and 4 from 10 to 12.

Endpoints: obviously effective: great improvement of appetite and disappearance of repeated respiratory infection following one to two treatment periods; ‚ effective: improvement of appetite and reduction of repeated respiratory infection (less than once each month) following one to two treatment periods; ƒ ineffective: no improvement of appetite and more than one respiratory infection following one to two treatment periods. The observations shall be made in the three-month follow-up studies after the treatment is completed.

Dosage and administration: 2 grams for those aged below 3, 3 grams for those aged 3 to 7, 6 grams for those aged over 7. Take orally once or twice each day.

Treatment period and requirements: generally one or two months as one treatment period, use of health food for a few treatment periods. Those using the food for less than one treatment period are not included. Avoid using any other drugs when taking the food, especially appetite stimulators.

Clinical findings: 85 cases are classified according to the criteria:

Obviously effective: 29 cases, or 34.0%

Effective: 53 cases, or 62.0%

Ineffective: 3 cases, or 4.0%

The total effectiveness rate is 96%.

Analysis of efficacy of the health food upon child anorexia and repeated respiratory infection

There are five symptoms for child anorexia and repeated respiratory infection: Poor appetite. 85 patients have the symptom before the treatment, 73 patients are improved following the treatment, the effectiveness rate being 85.8%. ‚ Repeated infection of the respiratory tract. 85 patients have the symptom before the treatment, 66 patients are improved following the treatment, the effectiveness rate being 77.6%. ƒ Low weight. 85 patients have the symptom before the treatment, 62 patients are improved following the treatment, the effectiveness rate being 72.9%. „ 75 patients have hemoglobin below 11.5 g% before the treatment and 70 patients over 11.5 g% following the treatment, the effectiveness rate being 93.3%. … Low content of zinc in hair (the normal being 90 m g/g). Hair of 22 patients are tested before treatment; only one is 90 m g/g while the rest are below normal, the average being 52.69 m g/g. 15 patients are over 90 m g/g following the treatment, the effectiveness rate being 68.1%. Zinc in hair is increased to 120.89 m g/g on the average.

Statistical analysis of the findings concerning weight, hemoglobin and zinc in hair reveals that the health food is significantly effective in the treatment of child anorexia and repeated respiratory infection and improvement in weight, hemoglobin and zinc in hair.

Note: weight (kg), hemoglobin (g%), zinc in hair (m g/g)

Efficacy of the health food upon child postinfectious syndrome of weakened spleen

1. Recruitment and Clinical observations

(1) Patients with a case history of acute or subacute infections with or without fever, still having symptoms of weakened spleen when infections are cured or almost cured.

(2) Patients suffering from anorexia, fatigue, sweating, thirst, abnormal excretion, poor sleeping, nervousness, teeth gnashing, sleepiness, pain in abdomen, etc.

(3) Patients suffering from emaciation, thinness, shortness, pale, yellow hair, white hair, spots, sore throat, enlargement of tonsils and lymph nodes, pathological swelling, heart murmur, slight-colored tongue, spotted tongue, white and greasy tongue,yellow and greasy tongue.

(4) Physical examinations reveal slight anemia, slight rise of count of WBC and Neutrophil, lower diastase in urine, lower cellular immunity, lower immunoglobulin IgG and IgA, increased immunocomplex in blood, and much lower content of copper in hair as well as low content of zinc and iron in hair.

Patients who meet the requirement of item (1) and bear more than three symptoms of items (2) and (3) and have at least one positive result in physical examinations are recruited as patients of postinfectious syndrome of weakened spleen.

Take care to remove other diseases of similar symptoms such as certain chronic infections.

Clinical data

General information

94 males and 56 females

Age: 76 patients 1 and 3, 56 between 4 and 6, 13 between 7 and 9, 5 between 10 and 12.

History: 32 patients with a case history of half a year, 37 of one year, 31 of 3 years, 8 of 4 years, 7 of 5 years, 3 of 6 years, 1 of 10 years.

Efficacy criterion:

Obviously effective: disappearance of main symptoms, normal endpoints in laboratory test on the whole, increase of weight, no recurrence of infections during treatment.

Effective: visible improvement of main symptoms, improved endpoints in laboratory test, at most one infection during treatment with quick recovery.

No change or worsening of symptoms, more than one infection during treatment which does not recover soon.

Typical cases

1. Yu Zhen, female, 2 years old. In 1993, she suffered from frequent coughs and fever, as much as several times every month. After being treated with antibiotics for about half a year, the child was of unstable condition and low appetite. Physical examinations revealed that she was pale, emaciated, with low weight (10 kg), sore throat (+), enlargement of tonsils (level I), increased breath sounds of both lungs, heart (-), soft abdomen, subcutaneous fat of 0.4 cm thick, hemoglobin of 10 g%, and zinc in hair of 45 PPM/g. Takes the health food orally twice a day. Given no other drugs; instead, given instructions on diet. Two weeks later, the child had significant improvement of appetite, no cough or fever and better complexion. Proceed to take the health food; four weeks later, the child had normal appetite and conditions. Proceed to take the health food for two months continuously; the child was of rosy cheeks, high spirits and good appetite. Weight was 12 kg, subcutaneous fat 1.2 cm and hemoglobin 11.5 g/%. Three months later, zinc in hair was found to be 101 PPM/g.

2. Huang Bing, male, 3 years old. In 1993, he was diagnosed with repeated respiratory infection, which occurred once or twice each month, accompanied by 39 to 40^oC fever. For two years, the child, who was of low appetite, had received frequent emergency treatment in hospitals and had been frequently injected with antibiotics. When coming to be treated, the child was coughing as a result of fever. He was treated with heat-removing and cough-relieving medicinal herbs as well as the health food (three times a day). Physical examinations revealed that he was of low weight (13 kg), sore throat (++), enlargement of tonsils (level I) with congestion, dry sputum sounds, heart (-), soft abdomen, subcutaneous fat of 0.5 cm thick, and zinc in hair of 45 PPM/g. One week later, it was found that the child was without fever, no cough, sore throat (+), enlargement of tonsils (level I) without congestion, no dry sputum sounds, and heart (-). Proceed to take the health food without using any other drugs. Two weeks later, the child returned to normal with improved appetite. Take the food for two months, during which he was examined every two weeks. Freed from respiratory infection, his weight was 14.5 kg, subcutaneous fat 1.2 cm, hemoglobin 11.5 g/% and zinc in hair 111 PPM/g. In the three-month follow-up study, the child was found to be normal.

3. Yang Ming, male, 6 years old. In 1988, he suffered from weakness, frequent colds and cough, repeated fever and low appetite. Physical examinations: pale, sore throat (+), enlargement of tonsils (level II) without congestion, some lymph nodes as large as soybeans, sputum sounds in both lungs, heart (-), soft abdomen, liver and spleen not touched, subcutaneous fat of 0.6 cm thick, body weight of 18.5 kg, hemoglobin of 11 g%, and zinc in hair of 82.6 PPM/g. Take the health food orally, three times a day. As he had cough and sputum, he was given cough syrup; no antibiotics were given. Two weeks later, the child had no cough or sputum. Proceed to take the health food; the child had normal improved appetite. Proceed to take the health food for two months continuously; the child was of rosy cheeks, no sore throat, tonsils (level I), smaller lymph nodes, lung & heart (-), subcutaneous fat of 1.2 cm, body weight of 20.3 kg, hemoglobin of 12.6 g/% and zinc in hair of 183 PPM/g. In the three-month follow-up studies, the child had no more respiratory infection.

Components of the health food SATTVA ANNAM

1. The health food contains proteins, amino acids, sugar, lactones, organic acids, coumarin, saponin, triterpene, steroids, aldehyde, etc. The health food is also rich in nutrition.

2.Analysis of proteins and amino acids

The biuret reaction essay finds that the health food contains 1.47% water soluble proteins.

The amino-acid analyzer finds that the total free amino acids is 0.1039%.

Fifteen amino acids were found in the health food, as we lacked sufficient standard amino-acid reagents (see table three). The acids of highest amount are TYR (227.26 ppm) and PHE (215.25 ppm), both as components of thyroxine. The health food contains many amino acids vital to the human body such as PHE, LYS, MET, LEU, ILE and VAL (the total being 600.4 ppm). These amino acids, not produced in the human body, can only be acquired through foods. Foods rich in amino acids are beneficial to children. The most important amino acid is lysine (LYS), but the content of LYS is lowest in rice, wheat and corn, and thus Lysine is referred to as estricted amino acid”. The health food contains much LYS supplementing nutrition.

3. Saccharides measurement

Saccharides in the health food include reducing sugar, polysaccharose and polysaccharides.

The health food is produced with various edible fungi, with the active components being the polysaccharides of fungi. According to recent research, polysaccharides of fungi prevent tumors, promote gastric and intestinal mucosal barriers, relieve ulcers and enhance immunity.

4.Analysis of microelements

Microelements such as iron, copper, zinc and manganese are essential to the human body for the formation of ferritin and copper-protein. Zinc and copper form various metalloenzymes involved in enzyme reactions vital to growth. Manganese, activating many enzymes, is important in the metabolism of carbohydrates, fats and proteins. Lack of microelements in the human body leads to microelement deficiency, which is especially harmful to children. Deficiency in iron, as well as copper or zinc, will result in anemia. Copper deficiency leads to Menkes’ syndrome and Wilson’ syndrome. Zinc deficiency leads to enteric acro-dermatitis, enlargement of the liver, Turner syndrome, dwarfism and low appetite. According to the American Academy of Science, the daily minimum intake of zinc should be 3 to 5 mg for babies, 10 mg for children aged between 1 and 10, 15 mg for those aged between 11 and 51, 20 mg for pregnant women and 25 mg for breast-feeding women. Japan proposes that the daily intake of copper should be 80  g/kg for infants and 40  g/kg for children. The US RDA believes that the daily intake of iron should be 1.0 mg/kg for term infants, 2.0 mg/kg for preterm infants, 15 mg/day for those aged 1 to 3, 10 mg/day for those aged 4 to 10, and 18 mg/day for those aged 11 to 18. It is imperative to provide children with health foods rich in essential elements such as iron and copper.

Note 1: Symbols such as “-, I, II, III, IV, +” are used to express level of diseases. “-” refers to negative or normal, I-IV” refers to disease level, from slight to serious, “+” refers to disease.

Note 2: Wide-spectrum: there are several immune systems in the human body such as the reticuloendothelial system, the lymphatic system, the humoral immune system; edible fungi are beneficial to all of the immune systems.